Sanger Sequencing

Amplicon sequencing using Sanger sequencing involves the PCR amplification of targeted DNA regions, followed by sequencing the amplified products to identify specific genetic variants or microbial taxa. This method provides high accuracy and is commonly used for applications such as mutation detection, genetic characterization, and studying microbial diversity, making it a reliable choice for focused genomic analyses. 

Microbiome identification using Sanger sequencing involves amplifying specific genomic regions, such as the 16S rRNA gene, to characterize microbial communities. This method provides accurate species-level identification by sequencing the amplified DNA fragments. Sanger sequencing is suitable for analyzing a limited number of samples, offering insights into microbial diversity and composition within a specific environment.