Whole genome sequencing (WGS) is a comprehensive method that determines the complete DNA sequence of an organism's genome, encompassing both coding and non-coding regions. Utilizing next-generation sequencing (NGS), WGS identifies a wide range of genetic variations, including single nucleotide variants (SNVs) and structural variants. It supports applications in clinical diagnostics, personalized medicine, and evolutionary research by providing a complete genomic overview.
Whole transcriptome sequencing (RNA-seq) is a high-throughput technique that analyzes the complete set of RNA transcripts expressed in a cell or tissue. Using next-generation sequencing (NGS), it captures all types of RNA, including mRNA and non-coding RNAs, enabling quantitative assessment of gene expression, alternative splicing, and transcriptome variations. This approach provides insights into cellular function and disease mechanisms.
Microbiome sequencing analyzes the genetic material of microbial communities in various environments, such as the human gut. Utilizing techniques like 16S rRNA gene sequencing or whole metagenomic sequencing, it identifies and quantifies microbial species and functional genes, providing insights into microbial diversity, ecology, and their roles in health and disease.
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